Further studies on the structure of bovine enterovirus particles. by Elizabeth Mary Hoey

Cover of: Further studies on the structure of bovine enterovirus particles. | Elizabeth Mary Hoey

Published .

Written in English

Read online

Edition Notes

Thesis (Ph. D.)--The Queen"s University of Belfast, 1974.

Book details

The Physical Object
Pagination1 v
ID Numbers
Open LibraryOL19187606M

Download Further studies on the structure of bovine enterovirus particles.

Enterovirus E (formerly bovine enterovirus (BEV)) is a picornavirus of the genus virus may also be referred to as enteric cytopathic bovine orphan virus (ECBO). It is endemic in cattle populations worldwide, and although normally fairly nonpathogenic, it can cause reproductive, respiratory, or enteric disease – particularly when the animal is concurrently infected with Family: Picornaviridae.

In Fenner's Veterinary Virology (Fourth Edition), Bovine Enterovirus. Bovine enteroviruses and bovine rhinoviruses are ubiquitous in cattle populations, and both viruses were recently re-classified within the genus are two species and approximately serotypes of human rhinovirus but, among domestic animals, rhinoviruses are recognized only in cattle.

Introduction. Bovine enterovirus is a small RNA picornavirus causing reproductive, gastrointestinal and respiratory disease in cattle. Most have a low virulence. The enteroviruses multiply primarily in the gastrointestinal tract but also in the muscles and nervous tissue.

There are 10 recognised serotypes within 2 serogroups. BEV is also often found concurrently with other diseases, including. Introduction. Bovine enterovirus (BEV) is a member of the picornavirus family. BEV contains ∼ nt single-stranded (ss) RNA of positive polarity with poly(A) tail at its 3′ end as a genome enclosed in a nonenveloped icosahedral capsid, which is composed of 60 copies of each of four proteins, VP1, VP2, VP3 and VP4 (Hoey and Martin,Todd and Martin,Earle et al., ).Cited by: 6.

The sequence variability within the BEV population was remarkably high, suggesting interesting implications for viral evolution. Sequencing and analysis of the VP1 region of the viral genome should be useful to further characterize these isolates and to confirm their serotype, as has been reported in other enterovirus typing studies ().Cited by: Background.

Bovine enterovirus (BEV) is a single positive-stranded RNA virus belonging to the genus Enterovirus within family viral particle is composed of a small, non-enveloped and icosahedral virion and k-base genome containing a single open reading frame (ORF) flanked by untranslated regions (UTRs) at the 5′ and 3′ by: 7.

Virology Structure. Picornaviruses are non-enveloped, with an icosahedral capsid. The capsid is an arrangement of 60 protomers in a tightly packed icosahedral structure. Each protomer consists of 4 polypeptides known as VP (viral protein) 1, 2, 3 and 4. VP2 and VP4 polypeptides originate from one protomer known as VP0 that is cleaved to give the different capsid : Picornavirales.

Extensive structural and biochemical studies have identified five distinct particles that represent the lifecycle of a human enterovirus: putative procapsid, provirion, mature infectious virus. A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text.

Background: Bovine enterovirus (BEV) belongs to the species Enterovirus E or F, genus Enterovirus and family Picornaviridae. Although numerous studies have identified BEVs in the feces of cattle with diarrhea, the pathogenicity of BEVs remains unclear.

Previously, we reported the detection of novel kobu-like virus in calf feces, by metagenomics. Bovine enterovirus (BEV) belongs to the species Enterovirus E or F, genus Enterovirus and family Picornaviridae. Although numerous studies have identified BEVs in the feces of cattle with diarrhea, the pathogenicity of BEVs remains unclear.

Previously, we reported the detection of novel kobu-like virus in calf feces, by metagenomics analysis. In the present study, we identified a novel BEV in Cited by: 7. A cytopathic virus was isolated using Madin-Darby bovine kidney (MDBK) cells from lung tissue of alpaca that died of a severe respiratory infection.

To identify the virus, the infected cell culture supernatant was enriched for virus particles and a generic, PCR-based method was used to amplify potential viral sequences.

Genomic sequence data of the alpaca isolate was obtained and compared with. Enterovirus A71 Further studies on the structure of bovine enterovirus particles.

book infection has grown to become a serious threat to global public health. It is one of the major causes of hand, foot, and mouth disease (HFMD) in infants and young children. EV-A71 can also infect the central nervous system (CNS) and induce diverse neurological complications, such as brainstem encephalitis, aseptic meningitis, and acute flaccid paralysis, or even by:   Through the use of immune sera prepared from chickens, the crossserological study of 22 prototypes of bovine enteroviruses isolated in different parts of the world, allowed us to identify two serological groups each of which contained 11 viruses.

The viruses of the first group are closely antigenically related although they can be distinguished in “broad” and “specific” variants, the Cited by: lished and used to classify bovine enteroviruses to BEV-A (currently named enterovirus E) and BEV-B (currently named enterovirus F), where different serotypes/genotypes were further divided for either enterovirus E or enterovirus F [27,28,29].

In this study, we reported the File Size: 3MB. These permits may be required for shipping this product: USDA APHIS VS or A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment.

The Application Form VS (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS or A permit. Bovine enteroviruses (BEV) are members of the genus Enterovirus in the family Picornaviridae.

They are predominantly isolated from cattle feces, but also are detected in feces of other animals, including goats and deer. These viruses are found in apparently healthy animals, as well as in animals with clinical signs and several studies reported recently suggest a potential role of BEV in Cited by: 2.

Measuring parameters of Bovine Enterovirus infection 1. 1 Lab report2- Bovine Enterovirus MatthewDower- Measuring parameters of Bovine Enterovirus infection Abstract The purpose of this experiment is to measure the parameters of bovine enterovirus infections.

Flavonoids are natural biomolecules that are known to be effective antivirals. These biomolecules can act at different stages of viral infection, particularly at the molecular level to inhibit viral growth. Enterovirus A71 (EV-A71), a non-enveloped RNA virus, is one of the causative agents of hand, foot and mouth disease (HFMD), which is prevalent in : Salima Lalani, Chit Laa Poh.

However, further studies are needed to verify if the capsid proteins produced by calpains could be used in the production of pentamers and subsequently in the assembly of infectious virions.

Interestingly, it has been shown earlier with foot-and-mouth disease virus that even longer amino acid segments can be attached to VP1 without hindrance in Author: Mira Laajala, Minna M. Hankaniemi, Juha A.

Määttä, Vesa P. Hytönen, Olli H. Laitinen, Varpu Marjo. Foot-and-mouth disease (FMD) has been recognized in printed records dating from the sixteenth century, and since the eradication of rinderpest (cattle plague) in the early part of the twentieth century it has been rec- nized as the most important and feared disease of cattle and other dom- tic livestock.

The beginning of the twenty-first century brought the worst outbreak of FMD ever. (D) X-ray structure of the enterovirus HRV-A, HRV) protease 2A pro (PDB no. 2HRV). The COOH-terminal domain coordinates a tightly bound Zn 2+ ion (pink sphere).

The catalytic triad is highlighted (red). (E) NMR structure of the soluble NH 2-terminal domain of the enterovirus HEV-C, poliovirus 1 3A protein (PDB no.

1NG7). Two genera of Picornaviridae— enterovirus and rhinovirus —have an identical morphology but can be distinguished based on clinical, biophysical, and epidemiological studies. Enteroviruses grow at a wide pH range (ie, ). After initial replication in the oropharynx, enteroviruses survive the acidic environment of the stomach.

The Enterovirus genus belongs to the Picornaviridae family, which includes poliovirus, Coxsackie virus, enteric cytopathogenic human orphan virus, and human enterovirus 71 (EV71). Non-enveloped enterovirus is spherical in shape, with a diameter ranging from 28 nm to 30 nm.

Its nucleocapsid is an icosahedron, and the viral capsid consists of 60 copies of capsid proteins arranged Cited by:   Electron Tomography Analysis of Tick-Borne Encephalitis Virus Infection in Human Neurons replication during viral infection will be a subject of our further studies Cited by: Studies have demonstrated that active milk protein and peptide compounds can be extracted from a variety of species including humans, bovine, porcine, mice and camel.

The main focus in this paper is the antiviral and immune regulating properties (Table 1) of milk proteins (Table 2) and their peptide derived fragments (Table 3).Cited by: 3. Bovine reproductive disease attributable to bovine herpes virus-1 (BoHV-1) was first described in Germany in the 19th century, being recognised primarily as the cause of infectious vulvovaginitis and balanoposthitis until the mids when a more virulent strain of the virus (BoHV) associated with respiratory disease (infectious bovine rhinotracheitis; IBR) emerged in the western Cited by: Human enteroviruses (EV) are members of the Enterovirus genus of the family Picornaviridae and are among the most common human viral infections.

These investigations had implications for all of virology because they indicated, first, that poliovirus (PV) grew in various tissue culture cells that did not correspond to the tissues infected during the human disease and, second, that PV destroyed Cited by: 3. Bovine Virus Testing PCR and fluorescent antibody panels of viruses used in our testing in compliance with the Code of Federal Regulations (9 CFR, parts and ), Animals and Animal Products.

The panel includes the 10 viruses listed below. Clients may request the inclusion of other viruses or modification of our standard protocols, as desired. They were discovered by Twort; further studies were made by DeHerelle. Experiment of Hershey & Chase is another example which confirms status of DNA as genetic material.

Safferman & Morris discovered 'Cyanophages' i.e., viruses which attack blue green algae like Lyngbya, Phormidium & Plectonema. They are also called LPP-1 on the basis of hosts. An interesting report from not only affirms the presence of these viruses in calf serum, and mentions the additional presence of bovine enterovirus-4, but also tells us that 25% of serum lots that were pre-tested by the suppliers and "considered to be free of known viral contaminants" were actually contaminated with bovine viruses (43).

Filovirus, any virus belonging to the family Filoviridae. Filoviruses have enveloped virions (virus particles) appearing as variably elongated filaments that are about 80 nm (1 nm = 10 −9 metre) in diameter and generally between and 1, nm in length.

The virions are pleomorphic (varying in shape) and contain a helical nucleocapsid, which consists of a protein shell, or capsid, and. Pathogenesis and Pathology of Bovine Pneumonia Roger J. Panciera, DVM, PhDa, Anthony W. Confer, DVM, PhDb,* Despite availability and use of many bovine respiratory pathogen vaccines and new antimicrobial drugs as well as greater understanding of the pathogenesis of bovine respiratory disease (BRD), pneumonia, ranging from subclinical to fatal.

erotypes 1 through 10 contain the viruses previously known as porcine enterovirus (PEV) serotypes and PTV were discovered after these viruses were transferred to the new Teschovirus genus, and should not be confused with the former PEV serotypes Teschovirus encephalomyelitis has traditionally been defined as the severe File Size: KB.

The family Filoviridae (/ ˌ f aɪ l oʊ ˈ v ɪr ɪ d iː /), a member of the order Mononegavirales, is the taxonomic home of several related viruses (filoviruses or filovirids) that form filamentous infectious viral particles and encode their genome in the form of single-stranded negative-sense RNA.

Two members of the family that are commonly known are Ebola virus and Marburg : Monjiviricetes. Introduction. Bovine Adenovirus belong to the Adenoviridae family. Most adenovirus infections in cattle involve either the respiratory (e.g. rhinitis) or gastrointestinal addition there have been reports of adenovirus associated with conjunctivitis, keratoconjunctivitis, and weak calf is now also thought to contribute to the disease complex Enzootic pneumonia of calves.

Human-Bovine Chimeric Respiratory Syncytial Virus (RSV) Vaccines Available for licensing and commercial development is a patent estate and related biological materials for making human-bovine chimeric virus particles for formulating live attenuated vaccines against human respiratory syncytial virus.

The inhibitory activity of secretions in cattle against foot and mouth disease virus. Ai, An ass~ of interferon produced in rhesus monkey and calf kidney tissue cultures using bovine enterovirus M6 aa challenge.

Further studies on the infection of pig kidney cells by : A. M Garland. bovine respiratory s to feed lot cattle and early lactation cattle because they are fed extra grain. Secondary to grain overload. the excessive grain leads to lacticacidosis.

Signs. Start studying Lecture 6&7- Bovine Respiratory Disease Complex and treatment. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Treatment of human excreta and animal manure (HEAM) is key in controlling the spread of persistent enteric pathogens, such as viruses.

The extent of virus inactivation during HEAM storage and treatment appears to vary with virus genome type, although the reasons for this variability are not clear.

Here, we investigated the inactivation of viruses of different genome types under conditions Cited by: In human and non-human primates, filoviruses (Ebola and Marburg viruses) cause severe hemorrhagic fever.

Recently, other animals such as pigs and some species of fruit bats have also been shown to be susceptible to these viruses. While having a preference for some cell types such as hepatocytes, endothelial cells, dendritic cells, monocytes, and macrophages, filoviruses are known to be Cited by: Aim: Objective of the present study was to isolate bovine herpes virus Type 1 (BHV-1) from semen of infected bull and to adapt it onto embryonated eggs and Madin–Darby bovine kidney (MDBK) cell line.

Further, the virus was identified by agar gel immunodiffusion (AGID) : Devprabha Samrath, Sanjay Shakya, Nidhi Rawat, Varsha Rani Gilhare, Fateh Singh.

57522 views Monday, November 23, 2020